Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Capítulos
- 00:05Título
- 00:36Growth and Harvest of Yeast Cells
- 03:14Cycloheximide Chase
- 05:23Post-alkaline Protein Extraction
- 06:51Results: Cue1 Is Required for Degradation of Deg1-Sec62
- 08:23Conclusion
Protein abundance reflects the rates of both protein synthesis and protein degradation. This article describes the use of cycloheximide chase followed by western blotting to analyze protein degradation in the model unicellular eukaryote, Saccharomyces cerevisiae (budding yeast).
