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冻干环介导等温扩增试剂的检测中的发展<em>贝氏柯克斯体</em
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
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冻干环介导等温扩增试剂的检测中的发展<em>贝氏柯克斯体</em

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07:27 min

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April 18, 2016

DOI:

10.3791/53839-v

DOI:
10.3791/53839-v

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07:27 min
April 18, 2016

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2 Views
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1Viral and Rickettsial Diseases Department,Naval Medical Research Center

Summary

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We described here a simple loop-mediated isothermal amplification (LAMP) method using lyophilized reagents for the detection of C. burnetii in patient samples.

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Lyophilized LAMP ReagentsCoxiella BurnetiiQ FeverDNA ExtractionLAMP ReactionPlasmid DNASerial Dilutions2x LAMP BufferBst DNA PolymeraseAgarose Gel ElectrophoresisReconstitution Buffer

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Chen, H., Ching, W. The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii. J. Vis. Exp. (110), e53839, doi:10.3791/53839 (2016).

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