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定性的および定量的検出のためのアッセイおよびタンパク質の抗菌薬のキャラクタリゼーション
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials
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定性的および定量的検出のためのアッセイおよびタンパク質の抗菌薬のキャラクタリゼーション

18,079 Views

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10:50 min

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April 10, 2016

DOI:

10.3791/53819-v

DOI:
10.3791/53819-v

•

10:50 min
April 10, 2016

•

10 Views
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1Department of Advanced Technology,The MITRE Corporation, 2Center for Biomedical Engineering,Brown University, 3The MITRE Corporation

Summary

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Here, we present protocols to rapidly screen and characterize enzymes for antimicrobial activity. The microslide diffusion assay and the dye-release assay utilize target bacterial substrates for qualitative and quantitative enzymatic activity evaluation.

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Farris, M. H., Ford, K. A., Doyle, R. C. Qualitative and Quantitative Assays for Detection and Characterization of Protein Antimicrobials. J. Vis. Exp. (110), e53819, doi:10.3791/53819 (2016).

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