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皮层肌动蛋白流通过结构照明显微镜数据的时空图像相关光谱量化性T细胞
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December 17, 2015
DOI:
1Department of Physics and Randall Division of Cell and Molecular Biophysics,King’s College London, 2Academic Department of Rheumatology, Centre for Molecular and Cellular Biology of Inflammation, Division of Immunology, Infection and Inflammatory Disease,King’s College London, 3ARC Centre for Advanced Molecular Imaging, Australian Centre for NanoMedicine,University of New South Wales Australia, 4Departments of Chemistry and Physic,McGill University
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To investigate flow velocities and directionality of filamentous-actin at the T cell immunological synapse, live-cell super-resolution imaging is combined with total internal reflection fluorescence and quantified with spatio-temporal image correlation spectroscopy.
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Ashdown, G., Pandžić, E., Cope, A., Wiseman, P., Owen, D. Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data. J. Vis. Exp. (106), e53749, doi:10.3791/53749 (2015).
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