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Entwicklung und Validierung einer quantitativen PCR-Methode für Equiden Herpesvirus-2-Diagnose in Atem Flüssigkeiten
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Entwicklung und Validierung einer quantitativen PCR-Methode für Equiden Herpesvirus-2-Diagnose in Atem Flüssigkeiten
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March 17, 2016
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Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.
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Hue, E. S., Fortier, C. I., Laurent, A. M., Quesnelle, Y. F., Fortier, G. D., Legrand, L. J., Pronost, S. L. Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids. J. Vis. Exp. (109), e53672, doi:10.3791/53672 (2016).
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