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פיתוח ותיקוף של שיטת ה- PCR כמותי עבור Equid ההרפס-2 אבחון בנוזלי הנשימה
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids
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פיתוח ותיקוף של שיטת ה- PCR כמותי עבור Equid ההרפס-2 אבחון בנוזלי הנשימה

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09:57 min

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March 17, 2016

DOI:

10.3791/53672-v

DOI:
10.3791/53672-v

•

09:57 min
March 17, 2016

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33 Views
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1LABÉO Frank Duncombe, 2Unité de Risques Microbiens (U2RM, EA 4655),Normandy University, 3Hippolia Foundation

Summary

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Here, we present a protocol for the development and validation of a quantitative PCR method used for the detection and quantification of EHV-2 DNA in equine respiratory fluids. The EHV-2 qRT-PCR validation protocol involves a three-part procedure: development, characterization of qRT-PCR assay alone, and characterization of the whole analytical method.

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Cite this Article

Hue, E. S., Fortier, C. I., Laurent, A. M., Quesnelle, Y. F., Fortier, G. D., Legrand, L. J., Pronost, S. L. Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids. J. Vis. Exp. (109), e53672, doi:10.3791/53672 (2016).

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