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A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster
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A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster
Capítulos
- 00:05Título
- 00:48MARCM Fly Generation and Timed Egg-Laying
- 02:11Heat Shock Induction
- 02:54Behavioral Testing
- 04:38Results: Lack of Response in Mechanosenstivive Mutants
- 05:10Conclusion
In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.
