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Immunology and Infection

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JoVE Journal Immunology and Infection
The Use of Flow Cytometry to Assess the State of Chromatin in T Cells
 

The Use of Flow Cytometry to Assess the State of Chromatin in T Cells

Article DOI: 10.3791/53533-v • 11:01 min • December 17th, 2015
December 17th, 2015 •
*1, *1, 1
1Department of Biology, Furman University
* These authors contributed equally

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Flow cytometry can be utilized to assess the state of chromatin within T cells. This protocol allows scientists to interpret evidence of chromatin decondensation during T cell activation demonstrated by an increase in the mean florescence intensity (MFI) of fluorescent Histone H3 antibodies

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Flow Cytometry Chromatin T Cells Immune Response Antigen Presentation T Cell Receptor Clonal Proliferation Chromatin Decondensation Histone Proteins Antibodies Na& 239;ve T Cells Activated T Cells Surface Markers Viability Fixable Dead Cell Stain Intracellular Staining Histone H3 Proteins Mean Fluorescence Intensity (MFI)
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