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ヒト末梢血の免疫表現型分析のための抗体のカクテルを準備する半自動のアプローチ
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
A Semi-automated Approach to Preparing Antibody Cocktails for Immunophenotypic Analysis of Human Peripheral Blood
DOI:

08:17 min

February 08, 2016

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Capítulos

  • 00:05Título
  • 00:58Operating Procedure
  • 06:25Results: Low Variability in Semi-automated Whole Blood Immunophenotyping
  • 07:04Conclusion

Summary

Traducción Automática

Whole blood Immunophenotyping is indispensable for monitoring the human immune system. However, the number of reagents required and the instability of specific fluorochromes in premixed cocktails necessitates daily reagent preparation. Here we show that semi-automated preparation of staining antibody cocktail helps establish reliable immunophenotyping by minimizing variability in reagent dispensing.

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