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Bacteriële Leaf Infiltratie Assay voor Beeldende karakterisatie van Plant Defense Reacties met behulp van de<em> Arabidopsis thaliana-Pseudomonas syringae</em> Pathosystem
JoVE Revista
Inmunología y contagio
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JoVE Revista Inmunología y contagio
Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
DOI:

11:50 min

October 01, 2015

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Capítulos

  • 00:05Título
  • 01:53Preparations Prior to Infiltration
  • 03:40Syringe Infiltration
  • 05:57Sampling Leaf Tissue and Quantifying Pathogen Growth
  • 09:11Results: Syringe Infiltration Assays can Quantitatively Assess Plant Immune Response
  • 10:33Conclusion

Summary

Traducción Automática

Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.

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