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In Vitro Generazione di Murine plasmacitoidi dendritiche Cellule dal comune linfoide Progenitori utilizzando l'AC-6 Alimentatore Sistema
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JoVE Revista Inmunología y contagio
In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
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In Vitro Generazione di Murine plasmacitoidi dendritiche Cellule dal comune linfoide Progenitori utilizzando l'AC-6 Alimentatore Sistema

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08:18 min

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November 23, 2015

DOI:

10.3791/53211-v

DOI:
10.3791/53211-v

•

08:18 min
November 23, 2015

•

6 Views
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1Graduate Institute of Immunology,National Taiwan University College of Medicine, 2Department of Biochemistry,Chang Gung University College of Medicine, 3Molecular Medicine Research Center,Chang Gung University College of Medicine

Summary

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In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.

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Plasmacytoid Dendritic CellsPDCsCommon Lymphoid ProgenitorsCLPsAC-6 Feeder SystemIn Vitro GenerationType I InterferonIFN-ICDCs

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Chang, S., Pai, L., Lee, C. In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System. J. Vis. Exp. (105), e53211, doi:10.3791/53211 (2015).

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