Myocardial infarction (MI) is not only followed by impaired cardiac function but also by apoptosis in the amygdala, a brain region involved in the behavioral consequences of MI. This protocol describes how to induce MI, collect amygdala tissue and measure caspase-3 activity, a marker of apoptosis, therein.
Gilbert, K., Godbout, R., Rousseau, G. Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction. J. Vis. Exp. (107), e53207, doi:10.3791/53207 (2016).