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Analyse von<em> Yersinia enterocolitica</em> Effektor Translokation in Wirtszellen unter Verwendung Beta-Lactamase-Effektor-Fusionen
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions
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Analyse von<em> Yersinia enterocolitica</em> Effektor Translokation in Wirtszellen unter Verwendung Beta-Lactamase-Effektor-Fusionen

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12:23 min

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October 13, 2015

DOI:

10.3791/53115-v

DOI:
10.3791/53115-v

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12:23 min
October 13, 2015

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1 Views
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1Institute for Medical Microbiology, Virology and Hygiene,University Medical Center Hamburg-Eppendorf, 2UKE Microscopy Imaging Facility,University Medical Center Hamburg-Eppendorf

Summary

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Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.

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Yersinia EnterocoliticaType III Secretion SystemEffector TranslocationBeta-lactamase FusionFRET ReporterQuantitative AnalysisHeLa CellsLaser Scanning Microscopy

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Wolters, M., Zobiak, B., Nauth, T., Aepfelbacher, M. Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions. J. Vis. Exp. (104), e53115, doi:10.3791/53115 (2015).

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