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الاستفادة المثلى من البروتينات التركيبية: تحديد فصل بين التبعيات مبينا هيكليا و / أو بقايا المرتبطة وظيفيا
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July 14, 2015
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Summary
Automatically generated
Synthetic protein sequences based on consensus motifs typically ignore co-evolving residues, that imply interpositional dependencies (IPDs). IPDs can be essential to activity, and designs that disregard them may result in suboptimal results. This protocol uses StickWRLD to identify IPDs and help inform rational protein design, resulting in more efficient results.
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Rumpf, R. W., Ray, W. C. Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues. J. Vis. Exp. (101), e52878, doi:10.3791/52878 (2015).
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