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동축 노즐을 사용하여 다 능성 줄기 세포의 알긴산 캡슐화
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Biología del desarrollo
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JoVE Revista Biología del desarrollo
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
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동축 노즐을 사용하여 다 능성 줄기 세포의 알긴산 캡슐화

12,373 Views

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07:13 min

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July 02, 2015

DOI:

10.3791/52835-v

DOI:
10.3791/52835-v

•

07:13 min
July 02, 2015

•

15 Views
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1Center for International Research on Integrative Biomedical System, Institute of Industrial Science,The University of Tokyo

Summary

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We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.

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Alginate EncapsulationPluripotent Stem CellsCo-axial NozzleSuspension CultureAggregationShear StressCapsule SizeAlg-Ca CapsuleAlg-PLL CoatingStem Cell NicheMass Production

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Cite this Article

Horiguchi, I., Sakai, Y. Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle. J. Vis. Exp. (101), e52835, doi:10.3791/52835 (2015).

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