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隔离和卫星细胞的表征鼠头Branchiomeric肌肉

JoVE Revista
Biología del desarrollo

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JoVE Revista Biología del desarrollo

Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

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隔离和卫星细胞的表征鼠头Branchiomeric肌肉

DOI:

10.3791/52802-v

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07:37 min

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July 20, 2015

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Paola L. Carvajal Monroy, Zipora Yablonka-Reuveni, Sander Grefte, Anne Marie Kuijpers-Jagtman, Frank A.D.T.G. Wagener, Johannes W. Von den Hoff

¹Department of Orthodontics and Craniofacial Biology, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center, ²Department of Biological Structure,University of Washington School of Medicine, ³Department of Biochemistry, Radboud Institute for Molecular Life Sciences,Radboud University Medical Center

Capítulos

00:05Título
01:33Head Muscle (Masseter, Digastric, and Levator Veli Palatini) Dissection
04:01Satellite Cell Isolation and Culture
06:18Results: Representative Myotube Generation from Rat Head Branchiometric Muscle Tissue
07:09Conclusion

Summary

Traducción Automática

这个协议描述了从一个9周龄大鼠branchiomeric头肌卫星细胞的分离。肌肉从不同的鳃弓起源。随后，卫星细胞在局部上光毫米大小来研究其分化培养。这种方法避免了卫星细胞的扩增和传代。

Tags

Keywords: Satellite Cells Head Branchiomeric Muscles Fibrosis Muscle Regeneration Cleft Palate In Vitro Culture Enzymatic Digestion Extracellular Matrix Gel

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