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JoVE Journal Developmental Biology
An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples
 

An Enzymatic Method to Rescue Mesenchymal Stem Cells from Clotted Bone Marrow Samples

Article DOI: 10.3791/52694-v • 08:58 min • April 12th, 2015
April 12th, 2015 •
*1, *1, 1, 2, 3, 4, 1
1Swiss Paraplegic Research, 2Orthopaedics and Spinal Surgery, Swiss Paraplegic Centre, 3Department of Neurosurgery, Lucerne Cantonal Hospital (LUKS), 4Section of Small Animal Surgery/Neurology, Vetsuisse Faculty, University of Zurich
* These authors contributed equally

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Resumen
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Mesenchymal stem cells are usually obtained from bone marrow and require expansion culture. When samples clot before processing, a protocol using the (enzymatic) thrombolytic drug urokinase can be applied to degrade the clot. Thus, cells are released and available for expansion culture. This protocol provides a rapid and inexpensive alternative to resampling.

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Enzymatic Method Rescue Mesenchymal Stem Cells Clotted Bone Marrow Samples Urokinase Degradation Of Clots Rapid And Inexpensive Alternative Bone Marrow Resampling Tissue Engineering Autologous Stem Cell Therapies Hematopoietic System Expansion Culture Direct Stem Cell Therapy Enzymatic Digestion Labor-intense Costly Thrombolytic Drug Metabolic Activity Chondrogenic Lineage Osteogenic Lineage Adipogenic Lineage
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