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छोटे अणु संग्राहक भेदभाव और इसके बाद ग्लूकोज भुखमरी का प्रयोग मानव प्रेरित pluripotent स्टेम सेल से उच्च शुद्ध cardiomyocytes की व्युत्पत्ति
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Capítulos
- 00:05Título
- 01:56Passaging of hiPSCs
- 04:52Cardiac Differentiation of Human iPSC
- 07:17Purification of Human Cardiomyocytes through Glucose Starvation
- 09:39Representative Analysis of Differentiated Cardiomyocytes
- 10:55Conclusion
Here, we describe a robust protocol for human cardiomyocyte derivation that combines small molecule-modulated cardiac differentiation and glucose deprivation-mediated cardiomyocyte purification, enabling production of purified cardiomyocytes for the purposes of cardiovascular disease modeling and drug screening.
