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Afleiding van zeer gezuiverde Cardiomyocyten van Human geïnduceerde pluripotente stamcellen Met behulp van kleine moleculen gemoduleerd Differentiatie en Latere Glucose Verhongering
JoVE Revista
Biología del desarrollo
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JoVE Revista Biología del desarrollo
Derivation of Highly Purified Cardiomyocytes from Human Induced Pluripotent Stem Cells Using Small Molecule-modulated Differentiation and Subsequent Glucose Starvation
DOI:

11:53 min

March 18, 2015

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Capítulos

  • 00:05Título
  • 01:56Passaging of hiPSCs
  • 04:52Cardiac Differentiation of Human iPSC
  • 07:17Purification of Human Cardiomyocytes through Glucose Starvation
  • 09:39Representative Analysis of Differentiated Cardiomyocytes
  • 10:55Conclusion

Summary

Traducción Automática

Here, we describe a robust protocol for human cardiomyocyte derivation that combines small molecule-modulated cardiac differentiation and glucose deprivation-mediated cardiomyocyte purification, enabling production of purified cardiomyocytes for the purposes of cardiovascular disease modeling and drug screening.

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