Journal
/
Medicina
/
Istituzione di umano epiteliali Enteroids e Colonoids dal tessuto intero e biopsia
JoVE Revista
Medicina
This content is Free Access.
JoVE Revista Medicina
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
Please note that all translations are automatically generated. Click here for the English version.

Istituzione di umano epiteliali Enteroids e Colonoids dal tessuto intero e biopsia

33,927 Views

•

06:33 min

•

March 06, 2015

DOI:

10.3791/52483-v

DOI:
10.3791/52483-v

•

06:33 min
March 06, 2015

•

13 Views
, , , ,
1Department of Pediatric General and Thoracic Surgery,Cincinnati Children’s Hospital Medical Center, 2Department of Medicine, Section of Gastroenterology and Hepatology,Baylor College of Medicine

Summary

Automatically generated

We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.

Explore More Videos

Human Epithelial EnteroidsHuman Epithelial ColonoidsIntestinal Stem CellsIntestinal EpitheliumGastrointestinal HomeostasisEx Vivo TechnologiesPrimary Cell Culture3D Epithelial OrganoidsCrypt-derived SystemBiopsy

Read Article

Cite this Article

Mahe, M. M., Sundaram, N., Watson, C. L., Shroyer, N. F., Helmrath, M. A. Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy. J. Vis. Exp. (97), e52483, doi:10.3791/52483 (2015).

Download .ris file

Copy

Share Video

.

Copy