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마우스의 담관 결찰 : 폐쇄성 담즙에 의한 염증성 간 손상과 섬유화의 유도
JoVE Revista
Medicina
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JoVE Revista Medicina
Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
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마우스의 담관 결찰 : 폐쇄성 담즙에 의한 염증성 간 손상과 섬유화의 유도

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08:56 min

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February 10, 2015

DOI:

10.3791/52438-v

DOI:
10.3791/52438-v

•

08:56 min
February 10, 2015

•

126 Views
, , , , , ,
1Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry,RWTH Aachen University, 2Institute for Laboratory Animal Science and Experimental Surgery,RWTH Aachen University, 3Department of Medicine III,RWTH Aachen University

Summary

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Disruption of bile flow results in severe inflammatory cholestatic liver injury with a characteristic time-dependent sequence of morphological alterations. Here we present a protocol for the surgical ligation of the common bile duct in mice that allows to induce a strong fibrotic response after 21 to 28 days.

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Bile Duct LigationMiceCholestasisLiver InjuryLiver FibrosisObstructive CholestasisBile Flow ObstructionSurgical TechniqueAnimal Model

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Tag, C. G., Sauer-Lehnen, S., Weiskirchen, S., Borkham-Kamphorst, E., Tolba, R. H., Tacke, F., Weiskirchen, R. Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis. J. Vis. Exp. (96), e52438, doi:10.3791/52438 (2015).

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