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Capítulos
- 00:05Título
- 01:00Sample Preparation
- 02:07Granzyme B Activity Assay
- 03:30Results: The Ala-Ala-Asp-S-Benzyl Substrate is Specific for Granzyme B
- 04:53Conclusion
We describe a simple, quantitative colorimetric assay that specifically measures the proteolytic activity of human, mouse or rat Granzyme B (GzmB). This protocol can be easily adapted for determining protease activity of other granule serine proteases by the hydrolysis of other synthetic peptide substrates with an appropriate recognition sequence.
