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레이디 얼 이동성 및 레트로 바이러스 복제 벡터 형질 도입, 비 접착 Alloresponsive T 림프구의 세포 독성 기능
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레이디 얼 이동성 및 레트로 바이러스 복제 벡터 형질 도입, 비 접착 Alloresponsive T 림프구의 세포 독성 기능
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February 11, 2015
DOI:
1Department of Neurosurgery,UCLA David Geffen School of Medicine, 2Department of Molecular and Medical Pharmacology,UCLA David Geffen School of Medicine, 3Department of Medicine,UCLA David Geffen School of Medicine, 4Brain Research Institute,UCLA David Geffen School of Medicine, 5Jonsson Comprehensive Cancer Center,UCLA David Geffen School of Medicine
Summary
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We describe a protocol to monitor radial mobility of non-adherent immune cells in vitro using a cell sedimentation manifold/slide apparatus. Cell migration is tracked on monolayers of tumor cells or on extracellular matrix proteins. Examination by light and fluorescence microscopy allows for observation of cell mobility and cytotoxic functionality.
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Erickson, K. L., Hickey, M. J., Kato, Y., Malone, C. C., Owens, G. C., Prins, R. M., Liau, L. M., Kasahara, N., Kruse, C. A. Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes. J. Vis. Exp. (96), e52416, doi:10.3791/52416 (2015).
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