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Bereiding en karakterisering van SDF-1α-Chitosan-Dextransulfaat Nanodeeltjes
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Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
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Bereiding en karakterisering van SDF-1α-Chitosan-Dextransulfaat Nanodeeltjes

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12:00 min

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January 22, 2015

DOI:

10.3791/52323-v

DOI:
10.3791/52323-v

•

12:00 min
January 22, 2015

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1Department of Medicine,Brigham and Women’s Hospital and Harvard Medical School, 2Laboratory for Biomaterials and Drug Delivery, Department of Anesthesiology, Division of Critical Care,Boston Children’s Hospital

Summary

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The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.

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SDF-1αChitosanDextran SulfateNanoparticlesPolyelectrolyte ComplexesProtein DeliveryStem Cell Homing

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Cite this Article

Bader, A. R., Li, T., Wang, W., Kohane, D. S., Loscalzo, J., Zhang, Y. Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles. J. Vis. Exp. (95), e52323, doi:10.3791/52323 (2015).

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