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דור של פלסמיד וקטורים לבטא חלבונים מתויג FLAG על פי תקנות ההסדרה של יזם אדם התארכות Factor-1α שימוש גיבסון עצרת
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February 09, 2015
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Summary
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Synthesis of custom plasmids is labor and time consuming. This protocol describes the use of Gibson assembly cloning to reduce the work and duration of custom DNA cloning procedure. The protocol described also produces reliable tagged protein constructs for mammalian expression at similar cost to the traditional cut-and-paste DNA cloning.
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Grozdanov, P. N., MacDonald, C. C. Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly. J. Vis. Exp. (96), e52235, doi:10.3791/52235 (2015).
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