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Fare Sırt Sütun Crush Yaralanmalarında mikroglia ve Makrofagların ile aksonal İlişkilerin Intravital Görüntüleme
JoVE Revista
Neurociencias
This content is Free Access.
JoVE Revista Neurociencias
Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury
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Fare Sırt Sütun Crush Yaralanmalarında mikroglia ve Makrofagların ile aksonal İlişkilerin Intravital Görüntüleme

11,171 Views

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08:43 min

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November 23, 2014

DOI:

10.3791/52228-v

DOI:
10.3791/52228-v

•

08:43 min
November 23, 2014

•

3 Views
, , ,
1Department of Neurosciences,Case Western Reserve University, 2Department of Biomedical Engineering,Case Western Reserve University, 3Department of Pediatrics,Case Western Reserve University

Summary

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Two-photon intravital imaging can be used to investigate interactions among different cell types in the spinal cord in their native tissue environment in a bone marrow chimeric animal with a dorsal column traumatic spinal cord crush injury.

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Intravital ImagingAxonal InteractionsMicrogliaMacrophagesMouseDorsal Column Crush InjurySpinal Cord InjuryTwo-photon MicroscopyBone Marrow ChimeraLaminectomy

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Cite this Article

Evans, T. A., Barkauskas, D. S., Myers, J. T., Huang, A. Y. Intravital Imaging of Axonal Interactions with Microglia and Macrophages in a Mouse Dorsal Column Crush Injury. J. Vis. Exp. (93), e52228, doi:10.3791/52228 (2014).

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