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Image-based flowcytometrie Techniek om Veranderingen in Granulocyt Functie Evalueer<em> In Vitro</em
JoVE Revista
Inmunología y contagio
This content is Free Access.
JoVE Revista Inmunología y contagio
Image-based Flow Cytometry Technique to Evaluate Changes in Granulocyte Function In Vitro
DOI:

06:13 min

December 26, 2014

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Capítulos

  • 00:05Título
  • 01:00Phagocytosis Assay Technique
  • 03:00Sample Analysis
  • 04:43Results: Representative Activated Granulocyte Subsets Images
  • 05:44Conclusion

Summary

Traducción Automática

This method demonstrates a technique for assessing granulocyte function by simultaneously measuring phagocytosis of bacteria and oxidative burst. Image-based flow cytometry allowed for the identification of three distinct subsets of activated granulocytes that all differed in their relative functional capacity.

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