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High Throughput Fluorometric Technik zur Bewertung der Makrophagen-Phagozytose und Aktinpolymerisation
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High Throughput Fluorometric Technik zur Bewertung der Makrophagen-Phagozytose und Aktinpolymerisation
Capítulos
- 00:05Título
- 01:38Plating and Treating Cells
- 02:16Opsonization of Flourescent Particles
- 03:36Phagocytosis
- 05:08Actin Staining
- 06:10Quantification
- 07:33Results: Confocal Microscopy of Phagocytosis and Actin Polymerization
- 08:47Conclusion
Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.
