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Een<em> Ex Vivo</em> Laser-induced Spinal Cord Injury Model Mechanismen van axonale degeneratie Beoordeel in real-time
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JoVE Revista Neurociencias
An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time
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Een<em> Ex Vivo</em> Laser-induced Spinal Cord Injury Model Mechanismen van axonale degeneratie Beoordeel in real-time

10,732 Views

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11:18 min

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November 25, 2014

DOI:

10.3791/52173-v

DOI:
10.3791/52173-v

•

11:18 min
November 25, 2014

•

2 Views
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1KY Spinal Cord Injury Research Center, Department of Neurological Surgery,University of Louisville, 2Hotchkiss Brain Institute, Department of Clinical Neurosciences,University of Calgary

Summary

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We present a protocol utilizing two-photon excitation time-lapse microscopy to simultaneously visualize the dynamics of axon and myelin injuries in real time. This proposed protocol permits studies of both intrinsic and extrinsic factors which can influence central myelinated axon fate after injury and contribute to permanent clinical disability.

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Cite this Article

Okada, S. L. M., Stivers, N. S., Stys, P. K., Stirling, D. P. An Ex Vivo Laser-induced Spinal Cord Injury Model to Assess Mechanisms of Axonal Degeneration in Real-time. J. Vis. Exp. (93), e52173, doi:10.3791/52173 (2014).

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