Journal
/
Biología
/
サブクローニングプラス挿入（SPI） - ジーンターゲティングベクターの迅速な構築のための新しい遺伝子改変方法
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Subcloning Plus Insertion (SPI) – A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
Please note that all translations are automatically generated. Click here for the English version.

サブクローニングプラス挿入（SPI） - ジーンターゲティングベクターの迅速な構築のための新しい遺伝子改変方法

16,150 Views

•

09:02 min

•

January 08, 2015

DOI:

10.3791/52155-v

DOI:
10.3791/52155-v

•

09:02 min
January 08, 2015

•

1 Views
, , , ,
1Department of Biochemistry,University of Leicester, 2Center for Fisheries, Environment and Aquaculture Sciences, 3ES Cell Facility, Centre for Core Biotechnology Services,University of Leicester

Summary

Automatically generated

Gene targeting methodologies can be used to generate transgenic mice with knockout, knock-in and tagged alleles. Here, we describe an improved method of recombineering in E. coli, that we term ‘subcloning plus insertion’, which can be used to generate custom gene targeting vectors rapidly.

Explore More Videos

SubcloningRecombineeringGene Targeting VectorHomologous RecombinationBacterial Artificial ChromosomeConditional Gene KnockoutKnock-in VectorBAC Reporter VectorSelection MarkerMultiplex ApproachPlasmid Gap Repair

Read Article

Cite this Article

Reddy, T. R., Kelsall, E. J., Fevat, L. M., Munson, S. E., Cowley, S. M. Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors. J. Vis. Exp. (95), e52155, doi:10.3791/52155 (2015).

Download .ris file

Copy

Share Video

.

Copy