The accessibility of reliable models to investigate vascular blood interactions in humans is lacking. We present an in vitro model of cultured primary human endothelial cells combined with human whole blood to investigate cellular interactions both in the blood (ELISA) and the vascular compartment (microscopy).
Nordling, S., Nilsson, B., Magnusson, P. U. A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium. J. Vis. Exp. (93), e52112, doi:10.3791/52112 (2014).