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Next-generation sequencing van het 16S ribosomaal RNA Gene Amplicons
JoVE Revista
Biología
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JoVE Revista Biología
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Next-generation sequencing van het 16S ribosomaal RNA Gene Amplicons

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10:24 min

August 29, 2014

DOI:

10:24 min
August 29, 2014

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Summary

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Characterizing microbial community has been a longstanding goal in environmental microbiology. Next-generation sequencing methods now allow for the characterization of microbial communities at an unprecedented depth with minimal cost and labor. We detail here our approach to sequence bacterial 16S ribosomal RNA genes using a benchtop sequencer.

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