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Sequenziamento di prossima generazione del 16S RNA ribosomiale Gene ampliconi
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August 29, 2014
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Characterizing microbial community has been a longstanding goal in environmental microbiology. Next-generation sequencing methods now allow for the characterization of microbial communities at an unprecedented depth with minimal cost and labor. We detail here our approach to sequence bacterial 16S ribosomal RNA genes using a benchtop sequencer.
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Sanschagrin, S., Yergeau, E. Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons. J. Vis. Exp. (90), e51709, doi:10.3791/51709 (2014).
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