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רצף של הדור הבא של 16S ריבוזומלי amplicons ג'ין RNA
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

רצף של הדור הבא של 16S ריבוזומלי amplicons ג'ין RNA

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10:24 min

August 29, 2014

DOI:

10:24 min
August 29, 2014

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Summary

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Characterizing microbial community has been a longstanding goal in environmental microbiology. Next-generation sequencing methods now allow for the characterization of microbial communities at an unprecedented depth with minimal cost and labor. We detail here our approach to sequence bacterial 16S ribosomal RNA genes using a benchtop sequencer.

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