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二光子<em> in vivoで</em>間引き頭蓋骨の準備を使用して、マウス皮質における樹状突起棘のイメージング
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

二光子<em> in vivoで</em>間引き頭蓋骨の準備を使用して、マウス皮質における樹状突起棘のイメージング

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09:53 min

May 12, 2014

DOI:

09:53 min
May 12, 2014

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Summary

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Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.

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