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双光子<em>在体内</em>树突棘中的鼠标皮质用稀疏的头颅成像准备
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

双光子<em>在体内</em>树突棘中的鼠标皮质用稀疏的头颅成像准备

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09:53 min

May 12, 2014

DOI:

09:53 min
May 12, 2014

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Summary

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Time-lapse imaging in the living animal provides valuable information on structural reorganization in the intact brain. Here, we introduce a thinned-skull preparation that allows transcranial imaging of fluorescently labeled synaptic structures in the living mouse cortex by two-photon microscopy.

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