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In vivo Optogenetic estimulação do sistema nervoso central de roedores
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January 15, 2015
DOI:
1Department of Psychiatry,University of Pittsburgh Medical Center, 2Department of Bioengineering,Stanford University, 3Department of Brain and Cognitive Sciences, Picower Institute for Learning and Memory,Massachusetts Institute of Technology, 4Department of Neurobiology and Behavior,Cornell University, 5Department of Psychiatry and Behavioral Sciences,Stanford University
Summary
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Optogenetics has become a powerful tool for use in behavioral neuroscience experiments. This protocol offers a step-by-step guide to the design and set-up of laser systems, and provides a full protocol for carrying out multiple and simultaneous in vivo optogenetic stimulations compatible with most rodent behavioral testing paradigms.
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Sidor, M. M., Davidson, T. J., Tye, K. M., Warden, M. R., Diesseroth, K., McClung, C. A. In vivo Optogenetic Stimulation of the Rodent Central Nervous System. J. Vis. Exp. (95), e51483, doi:10.3791/51483 (2015).
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