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高通量定量表达筛选与纯化应用到重组二硫键丰富的蛇毒蛋白产于<em> E。大肠杆菌</em
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Biología
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JoVE Revista Biología
High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli
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高通量定量表达筛选与纯化应用到重组二硫键丰富的蛇毒蛋白产于<em> E。大肠杆菌</em

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12:16 min

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July 30, 2014

DOI:

10.3791/51464-v

DOI:
10.3791/51464-v

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12:16 min
July 30, 2014

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9 Views
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1Architecture et Fonction des Macromolécules Biologiques (AFMB),Aix-Marseille Université, 2iBiTec-S, Service d’Ingénierie Moléculaire des Protéines (SIMOPRO),Commissariat à l’énergie atomique et aux énergies alternatives (CEA) Saclay, France

Summary

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一种协议，用于定量，高通量表达筛选，从小规模的大肠杆菌文化融合蛋白的纯化分析说明，并应用到的二硫键丰富的动物毒液蛋白靶点的表达。

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High ThroughputQuantitative Expression ScreeningRecombinant Disulfide-rich Venom ProteinsE. ColiStructural GenomicsDsbCDisulfide Bond FormationProtein ProductionTherapeutic Drug LeadsVenom ProteinsMass SpectrometryMicro-assays

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Saez, N. J., Nozach, H., Blemont, M., Vincentelli, R. High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli. J. Vis. Exp. (89), e51464, doi:10.3791/51464 (2014).

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