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An Innovative Method for Exosome Quantification and Size Measurement
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Biología
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JoVE Revista Biología
An Innovative Method for Exosome Quantification and Size Measurement

An Innovative Method for Exosome Quantification and Size Measurement

DOI:
10.3791/50974-v

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11:38 min

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January 17, 2015

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, , , , ,
1Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty,Heinrich-Heine-University Dusseldorf

Capítulos

  • 00:05Título
  • 01:51Exosome Preparation
  • 03:44Startup Procedure of the Particle Tracking Instrument
  • 06:25Measurement of Sample
  • 09:21Results: Interpretation of the Setup Possibilities and Resulting Data
  • 10:43Conclusion

Summary

Traducción Automática

Traducción Automática

A method for isolation of exosomes from whole blood and further analysis by nanoparticle tracking using a semi-automatic instrument is presented in this article. The presented technology provides an extremely sensitive method for visualizing and analyzing particles in liquid suspension.

Tags

Keywords: ExosomeNanoparticle Tracking AnalysisNTAUltracentrifugationExosome IsolationExosome QuantificationExosome Size MeasurementScanning Electron MicroscopySEMHuman PlasmaHealthDiseaseOncologyGynecologyCardiology

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