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用于可视化神经突在冷冻水合状态下使用低温电子断层扫描准备初级神经元
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February 12, 2014
DOI:
1Department of Molecular Physiology and Biophysics,Baylor College of Medicine, 2Department of Neuroscience,Baylor College of Medicine, 3Department of Neuroscience,University of California at San Diego, 4National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology,Baylor College of Medicine
Summary
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以保留神经流程超微结构分析，我们描述了一种协议，用于在电子显微镜网格后用快速冷冻原代神经元的电镀，得到的样品悬浮在一层玻璃的冰。这些样本可以检查与一个低温电子显微镜可视化结构在纳米尺度。
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Shahmoradian, S. H., Galiano, M. R., Wu, C., Chen, S., Rasband, M. N., Mobley, W. C., Chiu, W. Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography. J. Vis. Exp. (84), e50783, doi:10.3791/50783 (2014).
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