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Inmunología y contagio

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在纯化造血干/祖细胞识别DNA突变

JoVE Revista
Inmunología y contagio

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JoVE Revista Inmunología y contagio

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

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在纯化造血干/祖细胞识别DNA突变

12,847 Views

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11:06 min

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February 24, 2014

DOI:

10.3791/50752-v

DOI:

10.3791/50752-v

•

11:06 min

February 24, 2014

•

3 Views

Ziming Cheng¹, Ting Zhou^1,2, Azhar Merchant¹, Thomas J. Prihoda³, Brian L. Wickes^4,5, Guogang Xu², Christi A. Walter^2,5, Vivienne I. Rebel^1,2,5

¹Greehey Children’s Cancer Research Institute,UT Health Science Center at San Antonio, ²Department of Cellular and Structural Biology,UT Health Science Center at San Antonio, ³Department of Pathology,UT Health Science Center at San Antonio, ⁴Department of Microbiology,UT Health Science Center at San Antonio, ⁵Cancer Therapy and Research Center,UT Health Science Center at San Antonio

Summary

这里我们描述了对小数目使用了LacI转基因小鼠模型纯化造血干细胞的体内诱变法。该LacI的基因可以分离来确定频率，位置和类型的DNA突变的自发产生或暴露于基因毒素后。

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