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Immunology and Infection

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JoVE Journal Immunology and Infection
Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
 

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model

Article DOI: 10.3791/50741-v • 10:52 min • October 22nd, 2013
October 22nd, 2013 •
1, 1, 1, 1
1Faculty of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine

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Performing cell culture assays for investigating bacterial adhesion and invasion under aerobic conditions is usually unrepresentative of the in vivo environment. A Vertical Diffusion Chamber model allows study of interactions of the human pathogen Campylobacter jejuni with intestinal epithelial cells under more in vivo-like conditions, resulting in enhanced bacterial invasion.

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Keywords: Vertical Diffusion Chamber In Vitro Model Intestinal Epithelial Cells Campylobacter Jejuni Microaerobic Conditions Host-pathogen Interactions Bacterial Invasion Cell Culture
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