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Induction and Analysis of Epithelial to Mesenchymal Transition
JoVE Revista
Biología
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JoVE Revista Biología
Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

DOI:
10.3791/50478-v

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10:37 min

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August 27, 2013

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, , , ,
1Antibody Development Department,R&D Systems, Inc., 2Stem Cells and Developmental Biology Department,R&D Systems, Inc.

Capítulos

  • 00:05Título
  • 01:33Induction of EMT
  • 03:09Analysis of Protein Expression by Immunocytochemistry
  • 05:44Characterizing EMT-induced Human Cell Lines
  • 09:47Conclusion

Summary

Traducción Automática

Traducción Automática

A straightforward method is described for the induction of epithelial to mesenchymal transition (EMT) in a variety of cell types. Methods for the analysis of cells in EMT states by immunocytochemistry are included.

Tags

Epithelial To Mesenchymal TransitionEMTMorphogenesisFibrosisMetastasisIn Vitro InductionDrug DiscoveryDiagnosticImmunocytochemistryAntibody ArrayMigrationInvasion

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