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Immunhistochemische Analyse im Rat des Zentralnervensystems und peripheren Lymphknoten Gewebeschnitte
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Neurociencias
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JoVE Revista Neurociencias
Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
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Immunhistochemische Analyse im Rat des Zentralnervensystems und peripheren Lymphknoten Gewebeschnitte

16,784 Views

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09:11 min

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November 14, 2016

DOI:

10.3791/50425-v

DOI:
10.3791/50425-v

•

09:11 min
November 14, 2016

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10 Views
, , , , ,
1Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine,Karolinska Institutet, 2Department of Neuroimmunology, Center for Brain Research,Medical University of Vienna, 3Department of Medical Biochemistry and Biophysics, Vascular Biology Unit,Karolinska Institutet

Summary

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We here present an optimized, detailed protocol for double immunostaining in formalin-fixed, paraffin-embedded rat central nervous system (CNS) and peripheral lymph node (LN) tissue sections.

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ImmunohistochemistryRat Central Nervous SystemPeripheral Lymph NodeProtein LocalizationParaffin-embeddingAntigen RetrievalAntibodiesSignal DetectionLight Microscopy

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Adzemovic, M. Z., Zeitelhofer, M., Leisser, M., Köck, U., Kury, A., Olsson, T. Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections. J. Vis. Exp. (117), e50425, doi:10.3791/50425 (2016).

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