Journal
/
Inmunología y contagio
/
Isolation, Processing and Analysis of Murine Gingival Cells
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Isolation, Processing and Analysis of Murine Gingival Cells

Isolation, Processing and Analysis of Murine Gingival Cells

DOI:
10.3791/50388-v

•

09:47 min

•

July 02, 2013

•

, , ,
1Institute of Dental Sciences,Hebrew University – Hadassah Medical Center, 2Department of Periodontology,Hebrew University – Hadassah Medical Center

Capítulos

  • 00:05Título
  • 01:49Upper Gingival Excision
  • 03:24Gingival Processing
  • 04:57Extracellular and Intracellular Antibody Staining for Flow Cytometry Analysis
  • 06:58Representative Gingival Cell Analysis
  • 08:40Conclusion

Summary

Traducción Automática

Traducción Automática

This study describes an efficient technique to isolate and process gingival tissues from the mouse oral cavity in order to produce a single-cell culture. The resulting cells can be further used for flow cytometry analysis and molecular studies.

Tags

Murine Gingival CellsIsolationFlow CytometryMolecular StudiesImmune MechanismsPeriodontal DiseasesAlveolar BoneLangerhans CellsT Regulatory CellsOral Immune System

Article

Embed

Añadir a la lista de reproducción

Usage Statistics

Videos relacionados

Flow Cytometry Analysis of Immune Cells Within Murine Aortas

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells

Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Isolation and Activation of Murine Lymphocytes

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Read Article