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海馬スライス培養におけるシナプス蛋白のポストエンベディング免疫金ラベリング
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Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
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海馬スライス培養におけるシナプス蛋白のポストエンベディング免疫金ラベリング

16,928 Views

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13:50 min

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April 03, 2013

DOI:

10.3791/50273-v

DOI:
10.3791/50273-v

•

13:50 min
April 03, 2013

•

5 Views
, , ,
1Department of Cell Biology, Neurobiology and Anatomy,Medical College of Wisconsin , 2Department of Microbiology and Molecular Genetics,Medical College of Wisconsin

Tags

Immunogold LabelingPost-embeddingSynaptic ProteinsHippocampal Slice CulturesElectron MicroscopyUltrastructural LocalizationOsmium TetroxideUranyl AcetateTannic AcidAntigen DetectionStructural Preservation

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Zhong, L., Brown, J. C., Wells, C., Gerges, N. Z. Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures. J. Vis. Exp. (74), e50273, doi:10.3791/50273 (2013).

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