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Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

DOI:
10.3791/50146-v

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08:44 min

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September 24, 2013

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, , , ,
1Department of Neuropathology,Medical School Düsseldorf, 2Department of Molecular Genetics,Weizmann Institute for Science, 3Center of Behavioral Neuroscience,University of Düsseldorf

Capítulos

  • 00:05Título
  • 02:01In utero Electroporation (IUE)
  • 03:10Bioluminescence Live Imaging of the Enzymatic Luciferase Reaction
  • 05:47Analysis of Bioluminescence Images
  • 06:41Results: Luminescence Detection After Electroporation
  • 08:01Conclusion

Summary

Traducción Automática

Traducción Automática

Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.

Tags

Keywords: In Utero ElectroporationBioluminescence ImagingTransgenic RatsDISC1NeurodevelopmentBehavioral Neuroscience

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