Journal
/
Biología
/
基因捕获斑马鱼中使用的Gal4
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Gene Trapping Using Gal4 in Zebrafish
Please note that all translations are automatically generated. Click here for the English version.

基因捕获斑马鱼中使用的Gal4

17,935 Views

•

13:34 min

•

September 29, 2013

DOI:

10.3791/50113-v

DOI:
10.3791/50113-v

•

13:34 min
September 29, 2013

•

7 Views
,
1Department of Biology,Temple University

Summary

Automatically generated

本协议描述的使用GAL4-VP16为主要记者和GFP / RFP在斑马鱼次要记者基因陷阱插入突变的方法。约于一体的高表达F0鱼产量的基因陷阱后代共同表达GFP和RFP十名。筛选过程可以容易地缩放，以适应执行插入诱变屏幕实验室的规模。

Explore More Videos

Artificial IntelligenceGene TrapGal4ZebrafishInsertional MutagenesisFluorescent ReportersEnhancer TrapGene TrapGene Breaking Transposon (GBT)Gal4-VP16UAS:eGFPTransgene ExpressionSignal SequenceNuclear ProteinsCytoplasmic ProteinsPlasma Membrane Proteins

Read Article

Cite this Article

Balciuniene, J., Balciunas, D. Gene Trapping Using Gal4 in Zebrafish. J. Vis. Exp. (79), e50113, doi:10.3791/50113 (2013).

Download .ris file

Copy

Share Video

.

Copy