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为揭示复杂的大脑地形wholemount免疫组化
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JoVE Revista Neurociencias
Wholemount Immunohistochemistry for Revealing Complex Brain Topography
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为揭示复杂的大脑地形wholemount免疫组化

DOI:
10.3791/4042-v

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09:23 min

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April 05, 2012

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, , ,
1Department of Neuroscience,Albert Einstein College of Medicine, Yeshiva University , 2Department of Cell Biology and Anatomy and the Hotchkiss Brain Institute, Faculty of Medicine,University of Calgary

Capítulos

  • 00:05Título
  • 01:00Dissection of the Cerebellum
  • 02:05Processing the Tissue for Wholemount Staining
  • 06:10Imaging the Stained Tissue
  • 06:49Results: Zebrin II and CART Immunohistochemistry in Wholemount Cerebella
  • 08:55Conclusion

Summary

Traducción Automática

Traducción Automática

神经回路地形组织功能的车厢与特定的分子型材。在这里，我们提供一个多功能wholemount免疫组织化学染色方法为揭示全球脑地形使用的实际和技术步骤。我们证明了实用的使用方法很好理解的小脑细胞构筑和电路。

Tags

Wholemount ImmunohistochemistryBrain TopographyCerebellumCellular ArchitectureGene Expression DomainsProtein Expression DomainsMolecular CompartmentalizationAfferent FibersPatterning DefectsMouse CerebellumWholemount Staining ApproachProtein Expression PatternsZebrinII/aldolaseC ExpressionThree-dimensional ConformationAfferent ProjectionsComparative StudiesMolecular TopographyRat Cerebellum

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