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在OVO在鸡胚中脑多巴胺神经元发育中的基因功能研究电穿孔
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In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development
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在OVO在鸡胚中脑多巴胺神经元发育中的基因功能研究电穿孔

11,243 Views

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08:57 min

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August 03, 2012

DOI:

10.3791/4017-v

DOI:
10.3791/4017-v

•

08:57 min
August 03, 2012

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4 Views
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1Northwestern University Feinberg School of Medicine,Children’s Hospital of Chicago Research Center, 2Departments of Pediatrics, Neurology and Physiology,Northwestern University Feinberg School of Medicine

Summary

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评估的功能和在发展中脑多巴胺能神经元的基因调控，我们描述了一种方法，涉及<em>在OVO</em>鸡胚腹侧中脑多巴胺能神经元祖细胞质粒DNA结构的电。这种技术可以用来实现感兴趣的基因的高效表达，以研究脑发展和多巴胺神经元分化的不同方面。

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Yang, B., Geary, L. B., Ma, Y. In ovo Electroporation in Chick Midbrain for Studying Gene Function in Dopaminergic Neuron Development. J. Vis. Exp. (66), e4017, doi:10.3791/4017 (2012).

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